ИСТИНА

Lysozyme, the enzyme lysing microbial cells, can be considered as perspective antimicrobial agent. Medicines based on bacteriolytic enzymes should have high antimicrobial activity, be non-toxic, stable in long-term storage. The study aims to develop approaches to optimize the activity/stability of lysozyme for creating novel anti-infectives. Lysozyme (from chicken egg) has been investigated in its complexes with mucoadhesive and biodegradable polymers (chitosans of molecular weights 150, 400, and 600 kDa and alginic acids of different viscosities) as a basis for creating active and stable antimicrobial. Complexes of lysozyme with polymers were encapsulated by mucin (multilayer polyion complexes were formed). Obtained particles were characterized by physico-chemical and kinetic methods. The data obtained and reasons for observed phenomena are discussed.