ИСТИНА

Buckwheat (Fagopyrum) is a widely-cultivated pseudocereal belonging to eudicot family Polygonaceae. Due to its origin of radiation, North of India, its cultivation area is restricted by environmental conditions. By now the information about genetic control of stress response systems in buckwheat is almost absent. Recent advances of high-throughput sequencing and gene expression analysis offer new opportunities for the analysis of this important trait. Some essential abiotic stresses (heat, cold, rich light, wounding and darkness) were taken into analysis. Total RNA from cotyledons of stress-exposed and control plants was extracted and cDNA was sequenced using Illumina HiSeq2000 platform. After sequencing reads from all samples were assembled into general reference for further expression analysis. Differentially expressed genes identification was provided by CLC Genomics Workbench software [ver 5.0.1]. A set of genes expressed as a response on stress exposure was identified. Putative orthologs of Arabidopsis thaliana genes were found and their functional similarity between Arabidopsis and buckwheat is analyzed. Obtained data will promote the further analysis of abiotic stress response of buckwheat, functional analysis of differentially expressed genes and understanding of evolution of plant abiotic stress response systems.