ИСТИНА

The term “nanozyme” is apparently 5 years old. An interest to nanozymes is provided by the possibility for modelling catalytic pathways peculiar to natural enzymes. In addition, inorganic nanoparticles are characterized by both high stability and negligible cost peculiar to inorganic nanoparticles. In order to mimic natural enzyme peroxidase we synthesized nanoparticles of Prussian Blue (ferric hexacyanoferrate), the most advantageous electrocatalyst for hydrogen peroxide reduction, which has been discovered by us 25 years ago. The elaborated catalytic synthesis resulted in nanozymes with catalytic rate constants, which are significantly improved compared to natural enzyme peroxidase: for nanoparticles 200 nm in diameter, the turnover number is 300 times higher; for 570 nm diameter nanoparticles, it is 4 orders of magnitude higher. Comparing to the known peroxidase-like nanozymes with peroxidase-like activity, the advantages of the reported Prussian Blue nanoparticles are their true enzymatic properties: (1) enzymatic specificity (an absence of oxidase-like activity) and (2) an ability to operate in physiological solutions. The ultrahigh activity and enzymatic specificity of the catalytically synthesized Prussian Blue nanoparticles together with high stability and low cost, obviously peculiar to noble metal free inorganic materials, would allow the substitution of used nowadays natural and recombinant peroxidases, which are rather costly and not enough stable, in biotechnology and (bio)chemical analysis. We note that the synthesized Prussian Blue nanopzrticles are the only nanozymes suitable for medical diagnostics. References M.A. Komkova, E.E. Karyakina, A.A. Karyakin. Catalytically Synthesized Prussian Blue Nanoparticles Defeating Natural Enzyme Peroxidase. Journal of the American Chemical Society 140 (2018) 11302.