ИСТИНА

Determination of aromatic amino- and hydroxycompounds is an actual task of the environmental analytical chemistry. Often such a determination is performed using different colored derivatives. Thus, a search of new cheap and available approaches to a registration of the analytical response of colored samples is important for a development of simple and rapid analytical methods based on the signal measurement directly in the matrix of a sorbent or solid-phase reagent. A prospective way to solve this problem is using mini devices accumulating the new achievements of the optoelectronics and semiconductor industry. One of such devices is mini-spectrophotometer-monitor calibrator Eye-One Pro which is industrially manufactured by Swiss firm GretagMacbeth and serves as device for color control in printing industry as well as for automatic calibration of monitors, scanners and printers. From the view point of analytical chemistry it is important that this device can measure diffuse reflection coefficients in a range from 380 to 730 nm with a step of 10 nm as well as some colorimetric characteristics such as color coordinates in RGB, CMYK and Lab colorimetric systems. However, in spite of all the advantages of this device, the data on analytical applications of it are limited. In this work, the estimation of possibility of Eye-One Pro application for determination of organic compounds using diazotized polyurethane foam is given. The way of synthesis of such a polymeric chromogenic reagent was developed by us previously. 1-Naphthylamine, estrone and 4-aminophenol were chosen as analytes. They react with diazotized polyurethane foam with formation of intensely colored polymeric azo-dyes (460, 440 and 640 nm respectively). These substances were chosen because, on the one hand, development of approaches to their determination is essential, on the other hand, maxima of their analytical responses appear in different parts of spectrum and absorbance bands overlap almost the whole visible range. A comparison of the diffuse reflectance spectra recorded with Eye-One Pro and colorimeter “Spectroton” as well as Lab colorimetric coordinates has been made, and the dependence of shape and height of the absorbance band on the substance concentration in water solution has been investigated. It has been shown that using mini-spectrophotometer Eye-One Pro one can measure the signals of small (a few millimeters) samples that allow reaching an essential decrease in detection limit. The estimation of reproducibility for Eye-One Pro has been made. In the most cases relative standard deviations were less then 0.01. The approach used was shown to be prospective for the determination of aromatic amino- and hydroxycompounds in waters.