ИСТИНА

In this research, the diversity of MTB from Lake Beloe Bordukovskoe (Moscow region) was studied. Samples of water and sediment layers were collected from the lake and a microcosm was formed. The diversity of MTB in obtained microcosm was studied with help of a new separation method based on the use of MACS® Columns. This novelmethod is not limited by speed or motility of MTB and shows great results of MTB enrichment. Due to the fact that the ability to synthesize magnetosomes is not a taxonomic feature, it is a challenge to identifying MTB by the sequences of 16S rRNA genes. This occures because MAI genes could be transferred horizontally, therefore, inside one taxon there could be found MTB and non-MTB organisms. To avoid this difficulty, the primersystem based on mamK genes, which is specific for all of MTB, was developed.