ИСТИНА

Presence of different macromolecules such as plasma proteins and other synthetic molecules with high molecular weight in the environment of red blood cells (RBC) can alter red blood cells interaction and change the blood viscosity. This results in changes in reversible spontaneous aggregation and shear induced disaggregation processes, which are one of the major factors that affect hemorheology and blood microcirculation. This fact can be used in clinical practice for correcting rheological parameters by patient treatment. It is important to assess these alterations at different temperatures modeling their changes in association with changes of the systemic or local body temperature during inflammatory processes. It is known that the effect of macromolecules is dependent on their concentration both in the case of plasma proteins (fibrinogen, albumin, etc.) and in the case of other macromolecules (Dextran 40, 70, 150, 500, PVP, etc.). The exact role of each plasma protein in RBC aggregation is not fully understood so far. Sometimes the experiments are conducted using different techniques with whole blood samples, RBC suspensions in autologous serum or processed plasma, or pure solutions of plasma proteins/macromolecules yield somewhat controversial results. The influence of temperature on the aggregating and disaggregating forces between RBCs has not been studied in detail yet. Rheometry of human whole blood samples demonstrates a logarithmic dependence of the suspension viscosity on temperature at such shear rates that allow RBCs aggregation. We assumed that there might be an unaccounted temperature dependent synergetic effect of plasma proteins/macromolecules on RBC aggregation and interaction and, consequently, on the blood viscosity. The aim of this work was to assess the kinetics of RBC aggregation in-vitro in samples with varying plasma proteins/macromolecules content using different optical and rheometric techniques. This work was supported by RFBR grant № 17-02-01200-a.