ИСТИНА

In a recent publication, we have demonstrated for the first time that a conjugate of decyltriphenylphosphonium with plastoquinone (SkQ1) can serve as a carrier of adenosine 3,5-cyclic monophosphate (cAMP), one of the most important signaling compounds in living organisms. The data obtained on model liquid membranes and human platelets revealed the ability of SkQ1 to selectively transport cAMP, but not guanosine 3,5-cyclic monophosphate (cGMP), across both artificial and natural membranes. In particular, SkQ1 elicited translocation of cAMP from the source to the receiving phase of a Pressman-type cell, while showing low activity with cGMP. Importantly, only conjugate with plastoquinone, but not dodecyl-triphenylphosphonium lacking a quinone moiety, was effective in carrying cAMP. The SkQ1-induced transfer of cAMP, but not cGMP, across the plasma membrane of human platelets, from outside to inside the cells, was detected by phosphorylation of the vasodilator stimulated phosphoprotein. Possible impact of SkQ1 on cAMP signaling pathways in living cells will be discussed.