ИСТИНА

Presenting author email: tsarkova@colloid.chem.msu.ru Association colloids such as micelles, vesicles or aggregates of surfactants with synthetic- or bio-polymers play an important role in modern technologies including pharmaceutics and cosmetic, food, laundry and home care. We report a complex multicomponent system, containing ethoxylated anionic surfactants, anionic polyelectrolytes and enzymes, which posses a high efficiency to solubilize poorly water-soluble immobilized proteins (Figure 1A). By considering separate combinations of the system components at varied concentration regimes we addressed competitive interfacial and intermolecular interactions on different time- and length-scales. Using a set of methods such as dynamic tensiometry, solubilisation assay, molecular dynamic simulations we disclosed unexpected attractive interactions of fully neutralized polyacrylic acid (PAA) with like-charged surfactants in the presence of multivalent cations. A key contribution to the understanding and characterization of the macroscopic effect of enhanced solubilisation performance was gained by the analysis of dynamic processes at interfaces and of competitive binding interactions with Ca2+ ions in the multicomponent system (Figure 1B-C). In a narrow compositional range, the proposed bridging interactions via complexation with Ca2+ provide self-assembly of multicompartment dynamic soft nano-reactors with the solubilisation capacity exceeding that of conventional micelles [1]. Incorporation of a protease into such dynamic nanoreactors results in a synergistically enhanced cleaning performance. Apart from direct impact of these findings on the sustainability of laundry technology, revealing novel electrostatic association mechanisms and understanding interactions of hydrophobic species with biocompatible assemblies, like reported here compartmentalized surfactant/polymer/enzyme carriers, may give rise to new design approaches of delivery systems with possible biomedical applications.