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Catalytic Characteristics of Enzyme–Polyelectrolyte Complexes Based on Hexahistidine Containing Organophosphorus Hydrolaseстатья
Информация о цитировании статьи получена из
Scopus
Статья опубликована в журнале из списка Web of Science и/или Scopus
Дата последнего поиска статьи во внешних источниках: 19 сентября 2015 г.
- Авторы: Lyagin I.V., Efremenko E.N., Kabanov A.V.
- Журнал: Moscow University Chemistry Bulletin
- Том: 69
- Номер: 3
- Год издания: 2014
- Издательство: Allerton Press Inc.
- Местоположение издательства: United States
- Первая страница: 125
- Последняя страница: 130
- DOI: 10.3103/S0027131414030055
- Аннотация: A number of covalent and non-covalent polyelectrolyte complexes based on enzyme such as hexahistidine-containing organophosphorus hydrolase was developed in this work. Polyanions and polycations were used in the form of block-copolymers with poly(ethylene glycol). To obtain covalent complexes of the enzyme, different coupling agents (glutaric aldehyde, N-ethyl-N′-(3-dimethylaminopropyl)carbodiimide hydrochloride (EDC) and N-hydroxysulfosuccinimide sodium salt) were tried. The best catalytic efficiency of the action of the covalently bound complex ((2.8 ± 0.3) × 108 M−1 s−1) was obtained for samples with EDC. The modification of the protein surface was undertaken as an approach to its further biomedical application.
- Добавил в систему: Лягин Илья Владимирович