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Hybrid sol-gel bio-films: influence of synthetic parameters on behaviour and performance of entrapped His6-tagged organophosphorus hydrolaseстатья Научно-популярная статья
Статья опубликована в высокорейтинговом журнале
Информация о цитировании статьи получена из
Web of Science,
Scopus
Статья опубликована в журнале из списка Web of Science и/или Scopus
Дата последнего поиска статьи во внешних источниках: 20 сентября 2015 г.
- Авторы: Frančič N., Lyagin I.V., Efremenko E.N., Lobnik A.
- Журнал: Journal of Sol-Gel Science and Technology
- Том: 74
- Номер: 2
- Год издания: 2015
- Издательство: Springer Nature
- Местоположение издательства: Switzerland
- Первая страница: 387
- Последняя страница: 397
- DOI: 10.1007/s10971-014-3609-9
- Аннотация: The presented work describes the optimising of sol–gel process parameters including the aging time of the sol, the water/silane ratios, type of precursor [tetramethoxysilane (TMOS) or tetraethoxysilane (TEOS)], and the precursor ratios using organically modified silanes [methyltrimethoxysilane (MTMOS) and 3-glycidoxypropyltrimethoxysilane (GPTMS)], for the successful entrapment of hexahistidine-tagged organophosphorus hydrolase (His6-OPH) and its applicability during organophosphate detoxification processes. The prepared bio-composite films retained enzyme activity by up to 90 % with expected differences between the starting sols’ compositions. The obtained values of KM and Vmax for TMOS/MTMOS and TEOS/GPTMS bio-composites equalled 1.5 ± 0.1, 1.4 ± 0.3 mM, 0.40 ± 0.02 and 1.8 ± 0.2 μM/s for paraoxon, respectively, thus assuming effective application of such biocatalysts during the detoxifications of organophosphorus compounds. Furthermore, the bio-composite films showed enhanced pH and temperature stability with respect to the free enzymes as they can be easily separated from the reactive media, as well as having multiple reusages.
- Добавил в систему: Лягин Илья Владимирович