Novel molecular resources for single-specimen barcoding of enigmatic crustacean y-larvae

Dreyer, N.; Olesen, J.; Grygier, M.J.; Eibye-Jacobsen, D.; Savchenko, A.S.; Fujita, Y.; Kolbasov, G.A.; Machida, R.J.; Chan Benny, K.K.; Palero, F.

Авторы: Dreyer Niklas, Olesen Jørgen, Grygier Mark J., Eibye-Jacobsen Danny, Savchenko Alexandra S., Fujita Yoshihisa, Kolbasov Gregory A., Machida Ryuji J., Chan Benny K.K., Palero Ferran
Журнал: Invertebrate Systematics
Том: 38
Номер: 3
Год издания: 2024
Издательство: Commonwealth Scientific and Industrial Research Organization Publishing
Местоположение издательства: Australia
Первая страница: 1
Последняя страница: 14
DOI: 10.1071/is23018
Аннотация: Despite discovery more than 100 years ago and documented global occurrence from shallowwaters to the deep sea, the life cycle of the enigmatic crustacean y-larvae isincompletelyunderstood and adult forms remain unknown. To date, only 2 of the 17 formally describedspecies, all based on larval stages, have been investigated using an integrative taxonomicapproach. This approach provided descriptions of the morphology of the naupliar and cypridstages, and made use of exuvial voucher material and DNA barcodes. To improve our knowledgeabout the evolutionary history and ecological importance of y-larvae, we developed a novelprotocol that maximises the amount of morpho-ecological and molecular data that can beharvested from single larval specimens. This includes single-specimen DNA barcoding and dailyimaging of y-nauplii reared in culture dishes, mounting of the last naupliar exuviae on a slide as areference voucher, live imaging of the y-cyprid instar that follows, and fixation, DNA extraction,amplification and sequencing of the y-cyprid specimen. Through development and testing of asuite of new primers for both nuclear and mitochondrial protein-coding and ribosomal genes, weshowcase how new sequence data can be used to estimate the phylogeny of Facetotecta. Weexpect that our novel procedure will help to unravel the complex systematics of y-larvae andshow how these fascinating larval forms have evolved. Moreover, we posit that our protocolsshould work on larval specimens from a diverse array of moulting marine invertebrate taxa.
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Novel molecular resources for single-specimen barcoding of enigmatic crustacean y-larvaeстатья Исследовательская статья

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