Novel approach for downstream processing of phytosterol bioconversion

Khomutov, S.M.; Shutov, A.A.; Averin, A.D.; Dovbnya, D.V.; Donova, M.V.

Авторы: Khomutov Sergey M., Shutov Andrey A., Averin Aleksey D., Dovbnya Dmitry V., Donova Marina V.
Журнал: Journal of Chemical Technology and Biotechnology
Том: 98
Номер: 10
Год издания: 2023
Издательство: John Wiley & Sons Inc.
Местоположение издательства: United States
Первая страница: 2455
Последняя страница: 2461
DOI: 10.1002/jctb.7471
Аннотация: BACKGROUND: Downstream processing is an important part of industrial steroid biotechnology. The traditional recoverymethods require significant amounts of the organic solvents which are associated with environmental risks. There is a needfor environmentally friendly downstream processing which also may open the prospects for regeneration and repeated useof the costly materials.RESULTS: Microbial conversion of phytosterol to C17-ketosteroids in methylated cyclodextrin (mCD) solutions was studied as amodel bioprocess. Quantitative aspects of competitive complexation of mCD and natural ⊎-CD (⊎-CD) with steroids werestudied under different temperature modes and CD concentrations. The method of steroid recovery based on precipitationof insoluble ⊎-CD complexes ensured the complete isolation of androst-4-ene-3,17-dione, androsta-1,4-diene-3,17-dione, 9⊍-hydroxyandrost-4-ene-3,17-dione, 20-hydroxymethyl-pregna-1,4-dien-3-one and 3⊎-hydroxyandrost-5-en-17-one from aqueousmedia. Analysis of the 1H nuclear magnetic resonance (NMR) spectra showed a constant molar ratio of ⊎-CD to steroid equalto 2 in the obtained steroid ⊎-CD complexes. The extraction of the steroid from the ⊎-CD complex by small volumes of organicsolvent provided a quantitative yield. The method developed ensures regeneration and repeated use of the mCDs withoutdecrease of the basic bioconversion outputs, as was demonstrated in a series of phytosterol to androsta-1,4-diene-3,17-dionebioconversions by Mycolicibacterium neoaurum VKM Ac-1816D.CONCLUSIONS: Downstream processing based on manipulation of steroid solubility in aqueous cyclodextrin solutions is apromising alternative to traditional steroid extraction with organic solvents. This environmentally friendly approach ensuresre-use of materials without decrease in bioconversion outputs.
Добавил в систему: Аверин Алексей Дмитриевич

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