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Интеллектуальная Система Тематического Исследования НАукометрических данных |
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Phylogenomic insights on the evolution of Metchnikovellidsстатья
Дата последнего поиска статьи во внешних источниках: 14 августа 2017 г.
- Авторы: Nassonova E.S., Moreira D., Torruella G., Timpano H., Paskerova G., Smirnov A., Lopez-Garcia P.
- Журнал: Protistology
- Том: 10
- Номер: 2
- Год издания: 2016
- Издательство: Protozoological Society affiliated with RAS, Institute of Cytology RAS, Penza State University
- Местоположение издательства: Russia
- Первая страница: 52
- Последняя страница: 52
- Аннотация: Metchnikovellids constitute a group of hyperparasites that infect gregarines living in the gut of polychaetes and other marine invertebrates. Despite they were described in the late 19th century, they are poorly known and their phylogenetic affinities have remained elusive for a long time. Morphological studies suggested an evolutionary relationship with Microsporidia, a group of highly derived intracellular parasites known for its extreme metabolic and genomic simplification, including e.g. loss of the mitochondrion. Microsporidia together with Rozellida (Cryptomycota) and Aphelida form a monophyletic holomycotan clade, the superphylum Opisthosporidia. The first molecular phylogenetic analyses based on SSU rRNA and beta-tubulin genes of Metchnikovella incurvata, a parasite of the gregarine Polyrhabdina sp. from the gut of the polychaete Pygospio elegans, supported a close evolutionary relationship with microsporidia. However, unraveling the phylogenetic position of these organisms is difficult due to their high evolutionary rate. To improve the phylogenetic signal and ascertain the phylogenetic position of metchnikovellids, we applied a single-cell genomics approach to individual gregarine cells infected with M. incurvata. We generated genome data by multiple displacement amplification followed by direct HiSeq 2500 Illumina sequencing. After assembly, we mined the genome dataset in search of conserved genes. Preliminary phylogenomic analyses of 31 conserved genes confirm the phylogenetic placement of metchnikovellids at the base of Microsporidia and after the divergence of Mitosporidium daphniae, a microsporidia-like mitochondrion-bearing parasite. Further exploration of metchnikovellid genomes would allow determining the genes and traits involved in the evolution of extreme parasitism.
Прикрепленные файлы
| № | Имя | Описание | Имя файла | Размер | Добавлен |
|---|---|---|---|---|---|
| 1. | Nassonova_etal_Protist_2016.pdf | Nassonova_etal_Protist_2016.pdf | 358,9 КБ | 15 апреля 2017 [NassonovaES] |