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Chloride conducting light activated channel GtACR2 can produce both cessation of firing and generation of action potentials in cortical neurons in response to lightстатья
Информация о цитировании статьи получена из
Web of Science,
Scopus
Статья опубликована в журнале из списка Web of Science и/или Scopus
Дата последнего поиска статьи во внешних источниках: 11 апреля 2017 г.
- Авторы: Malyshev A.Y., Roshchin M.V., Smirnova G.R., Dolgikh D.A., Balaban P.M., Ostrovsky M.A.
- Журнал: Neuroscience Letters
- Том: 640
- Год издания: 2017
- Издательство: Elsevier BV
- Местоположение издательства: Netherlands
- Первая страница: 76
- Последняя страница: 80
- Аннотация: Optogenetics is a powerful technique in neuroscience that provided a great success in studying the brainfunctions during the last decade. Progress of optogenetics crucially depends on development of newmolecular tools. Light-activated cation-conducting channelrhodopsin2 was widely used for excitation ofcells since the emergence of optogenetics. In 2015 a family of natural light activated chloride channelsGtACR was identified which appeared to be a very promising tool for using in optogenetics experimentsas a cell silencer. Here we examined properties of GtACR2 channel expressed in the rat layer 2/3 pyra-midal neurons by means of in utero electroporation. We have found that despite strong inhibition thelight stimulation of GtACR2-positive neurons can surprisingly lead to generation of action potentials,presumably initiated in the axonal terminals. Thus, when using the GtACR2 in optogenetics experiments,its ability to induce action potentials should be taken into account. Our results also open an interestingpossibility of using the GtACR2 both as cell silencer and cell activator in the same experiment varyingthe pattern of light stimulation.
- Добавил в систему: Фельдман Татьяна Борисовна