Effect of dimerization on the catalytic properties of native and chimeric organophosphorus hydrolase determined by molecular modeling of the enzyme structureстатья
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Дата последнего поиска статьи во внешних источниках: 19 июля 2013 г.
Аннотация:The mechanisms of structural reorganization of a protein globule resulting in changes in the stability of enzyme depending on the pH of the medium were revealed by molecular dynamics modeling of the structure of the organophosphorus hydrolase (OPH) dimer. The same reorganization leads to changes in the substrate specificity of the enzyme depending on its genetic modification, which was experimentally confirmed. Based on the obtained theoretical data, it was concluded that the dimerization significantly affects the catalytic characteristics of the native form of OPH. An analysis of the whole set of theoretical and experimental data concerning the characteristics of the OPH chimeric forms suggests that their changes with respect to native OPH is a decrease in the level of dimerization of chimeric protein molecules.