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Formation of quasi-regular compact structure of poly(methacrylic acid) upon an interaction with alpha-chymotrypsinстатья
Информация о цитировании статьи получена из
Web of Science,
Scopus
Статья опубликована в журнале из списка Web of Science и/или Scopus
Дата последнего поиска статьи во внешних источниках: 18 июля 2013 г.
- Авторы: Kudryashova E.V., Gladilin A.K., Izumrudov V.A., van Hoek A., Visser A.J.W.G, Levashov A.V.
- Журнал: Biochimica et Biophysica Acta (BBA)/Protein Structure and Molecular Enzymology
- Том: 1550
- Номер: 2
- Год издания: 2001
- Первая страница: 129
- Последняя страница: 143
- DOI: 10.1016/S0167-4838(01)00274-6
- Аннотация: Structure and dynamic properties of free poly(methacrylic acid) (PMA) and PMA complexed with alpha -chymotrypsin (CT) were studied using the time resolved fluorescence anisotropy technique. We have found that the interaction of PMA with CT induces the formation of a quasi-regular structure of PMA. At a CT/PMA weight ratio of 4:1 the interaction with CT leads to formation of approximately four equal segments of polyelectrolyte, each binding one CT molecule and characterized by an independent rotational mobility. Increase of the CT/PMA weight ratio above 8:1 gives rise to the overall rotation of the whole enzyme-polyelectrolyte complex. In water-ethanol mixtures the mobility of PMA segments containing CT decreases and the structure of the complex becomes even more rigid due to enhancement of the electrostatic interaction between CT and PMA. Formation of the compact and quasi-regular structure of the complex is perhaps the main reason behind the enhancement of enzyme stability and suppression of enzyme aggregation in water-organic cosolvent mixtures. (C) 2001 Elsevier Science BN. All rights reserved.
- Добавил в систему: izumrudov