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Extensive hydrolysis of phosphonates as unexpected behaviour of the known His6-organophosphorus hydrolaseстатья Научно-популярная статья
Статья опубликована в высокорейтинговом журнале
Информация о цитировании статьи получена из
Web of Science,
Scopus
Статья опубликована в журнале из списка Web of Science и/или Scopus
Дата последнего поиска статьи во внешних источниках: 2 сентября 2016 г.
- Авторы: Lyagin I.V., Andrianova M.S., Efremenko E.N.
- Журнал: Applied Microbiology and Biotechnology
- Том: 100
- Номер: 13
- Год издания: 2016
- Издательство: Springer Verlag
- Местоположение издательства: Germany
- Первая страница: 5829
- Последняя страница: 5838
- DOI: 10.1007/s00253-016-7407-x
- Аннотация: The catalytic activity of hexahistidine-tagged organophosphorus hydrolase (His6-OPH) in hydrolytic reactions of methylphosphonic acid (MPA) and its monoesters and diesters being decomposition products of R-VX was demonstrated for the first time. The catalytic constants of enzyme in such reactions were determined. The mechanism of C–P bond cleavage in the MPA by His6-OPH was proposed. Such reaction was estimated to be carried out with the soluble and nanocapsulated forms of His6-OPH. His6-OPH was demonstrated to be capable of degrading the key organophosphorus components of reaction masses (RMs) that are produced by the chemical detoxification of R-VX and RMs are multi-substrate mixtures for this enzyme. The kinetic model describing the behaviour of His6-OPH in RMs was proposed and was shown to adequately fit experimental points during degradation of the real samples of RMs.
- Добавил в систему: Лягин Илья Владимирович
Прикрепленные файлы
| № | Имя | Описание | Имя файла | Размер | Добавлен |
|---|---|---|---|---|---|
| 1. | 10.1007_s00253-016-7407-x-1_stranitsa.pdf | 10.1007_s00253-016-7407-x-1_stranitsa.pdf | 459,4 КБ | 18 мая 2016 [Efremenko] |