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Monomolecular enzyme films stabilized by amphiphilic polyelectrolytes for biosensor devicesстатья
Информация о цитировании статьи получена из
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Web of Science
Статья опубликована в журнале из списка Web of Science и/или Scopus
Дата последнего поиска статьи во внешних источниках: 18 июля 2013 г.
- Авторы: Eremenko A., Kurochkin I., Chernov S., Barmin A., Yaroslavov A., Moskvitina T.
- Журнал: Thin Solid Films
- Том: 260
- Номер: 2
- Год издания: 1995
- Издательство: Elsevier Sequoia
- Местоположение издательства: Switzerland
- Первая страница: 212
- Последняя страница: 216
- DOI: 10.1016/0040-6090(94)06403-2
- Аннотация: This paper describes the use of new amphiphilic polyelectrolytes for protein immobilization. Monomolecular films of glucose oxidase (GO) and monoamine oxidase (MAO) stabilized by amphiphilic polyelectrolytes (polyethyleneimine and poly-4-vinylpyridine derivatives modified by lauryl chains) were formed on a water surface in a Langmuir-Blodgett trough. The compressed films containing the enzymes were transferred onto the surface of a polypropylene membrane of a Clark electrode according to the Langmuir-Schaefer method. The analytical responses of the resulting biosensors were linear over the range 1-10 mM of glucose and 8-100 μM of tyramine. Furthermore, direct functional coupling of GO and ferrocenecarboxylic acid in multilayer films stabilized by amphiphilic polyelectrolytes was demonstrated. The amperometric response of such a sensor was linear over the range 1-20 mM of glucose. The dependence of the kinetic parameters of the enzymes on the amphiphilic polyelectrolyte structures is discussed. © 1995.
- Добавил в систему: Курочкин Илья Николаевич