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Интеллектуальная Система Тематического Исследования НАукометрических данных |
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Electrochemical analysis of neuropathy target esterase activity by 1-methoxyphenazine methosulfate modified tyrosinase carbon paste electrode: progress in biomonitoring of organophosphate-induced delayed neurotoxicityдоклад на конференции
- Авторы: Sigolaeva L.V., Sokolovskaya L.G., Kurochkin I.N., Makhaeva G.F., Malygin V.V., Eremenko A.V.
- Конференция : The 17th European Conference on Solid-State Transducers "Eurosensors XVII" September 21 - 24
- Даты проведения конференции: 2003
- Тип доклада: не указан
- Докладчик: не указан
- Место проведения: Guimarães, Portugal, Portugal
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Аннотация доклада:
The 1-methoxyphenazine methosulfate modified tyrosinase graphite paste biosensor was developed. Electrode composition and measuring conditions were optimized allowing to detect phenol at -150 mV vs. Ag/AgCl reference electrode with sensitivity of 0.8 A/M*cm2 and the lower detection limit of 25 mM in a flow. The biosensor was applied for the assaying of the activity of toxicologically significant enzyme - neuropathy target esterase (NTE) produced phenol by the hydrolysis of the substrate, phenyl valerate. High sensitivity of the sensor enabled NTE to be assayed in whole blood, whereas the usual colorimetric assay is impossible. In vitro and in vivo correlation of NTE activity obtained in brain and whole blood show promise the biosensor NTE assay for whole blood as a biomarker of human exposure to neuropathic organophosphorus compounds.
- Добавил в систему: Сиголаева Лариса Викторовна