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Интеллектуальная Система Тематического Исследования НАукометрических данных |
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This work examines the fabrication regime and the properties of polymer-enzyme nanolayers deposited via layer-by-layer technology on the conductive surfaces (graphite, gold). The films were composed of linear homopolyelectrolytes or micelle-forming amphiphilic diblock copolymers (which form 1st layer of the polymer-enzyme coating) and enzymes tyrosinase and choline oxidase (which form 2nd layer of polymer-enzyme coating). Poly(2-(dimethylamino)ethyl methacrylate) (PDMAEMA) and poly(dimethyldiallylammonium chloride) (PDDA) were used as linear homopolyelectrolytes. Polybutadiene-block-poly(2-(dimethylamino)ethyl methacrylate) (PB-b-PDMAEMA) and poly(n-butyl methacrylate)-block-poly(2-(dimethylamino)ethyl methacrylate) (PnBMA-b-PDMAEMA) were used as diblock copolymers. The efficiency of the interaction of the copolymers with low charged and/or hydrophobic substrate (surface coverage and mass of the adsorbed substance) is inversely proportional to the charge of a macromolecule. In turn, the amount of the enzyme adsorbed and their activity are directly proportional to the amount and the charge of the preadsorbed polymer. The polymer/enzyme bilayers were tested as biosensors for phenol (for the films with tyrosinase) and choline (for the films with choline oxidase) and their activity and stability was found to be high.